Biography:

Will be updated.

Abstract:

With the observation of small objects, precise manipulation is also highly desirable, especially for three-dimensional manipulation of nanoparticles or biomolecules with a size of less than 100 nm [1]. Although optical tweezers have become powerful tools to manipulate microparticles and cells, they have limits when extended to the nanoscale because of the fundamental diffraction limit of light. The emergence of near-field methods, such as plasmonic tweezers and photonic crystal resonators, has enabled surpassing the diffraction limit. However, these methods are usually used for two-dimensional manipulation and may lead to local heating effects that will damage the biological specimens. Therefore, we propose a near-field technique that uses a photonic nano jet to perform the three-dimensional optical manipulation of sub-100-nm objects. With the photonic nano jet generated by a dielectric microlens bound to an optical fiber probe, three-dimensional manipulations were achieved for fluorescent nanoparticles as well as for plasmid DNA molecules [2]. Backscattering and fluorescent signals from the trapped targets were detected in real-time with a strong enhancement. The demonstrated approach provides a potentially powerful tool for nanostructure assembly, biosensing, and single-biomolecule studies.

Biography:

Apollonov V.V. is the leading specialist in the area of basic principles of creation and development of high power/energy laser systems and high power /energy radiation interaction with a matter.  He has made an outstanding input into creation and development of new branches of science - physical and technical fundamentals of high power/energy laser optics and adaptive optics, investigation of physical processes in a high volume self-controlled discharges, creation of high power/energy continuous wave, pulsed and high repetition rate pulse-periodic laser systems, high power/energy lasers radiation interaction with matter, application of  high power/energy lasers for effective  protection of valuable objects and water surface cleaning, medical applications. Recent results of his investigations of laser diode arrays phase-locking, mechanisms of shock waves merging for a rockets launch by high repetition rate pulse-periodic laser light and super long conductive

channel based on dust plasma by ablation are under very intensive implementation in our country and abroad.  He is the author of more than 1000 publications and patents.

          Apollonov V.V. is a high profile scientist and well known around all over the world, for more than 50 years of his international scientific activity, he has participated and organized more than 60 international conferences, symposiums and workshops, prepared 34 candidates and doctors of physics and mathematics.

          Apollonov V.V. is the member of European and American Physical Society, SPIE, AIAA, American Society for QE and the member of  specialized scientific council of Russia. He is a full member of Russian Academy of Natural Science, Academy of Engineering Sciences and Academy of Military science, laureate of State prize of USSR (1982), of  Russia (2001) and International prize ECOWORLD-2017.

Abstract:

Timing: 09:10:00 to 09:35:00 EDT 

The advent of the laser has placed stringent requirements on the fabrication, performance and quality of optical elements employed within systems for most practical applications. Their high power performance is generally governed by three distinct steps, firstly the absorption of incident optical radiation (governed primarily by various absorption mechanisms); secondly, followed by a temperature increase and response governed primarily by thermal properties and finally the elements thermo-optical and thermo-mechanical response, e.g., distortion, stress birefringenous fracture, etc. All of which needs to be understood in the design of efficient, compact, reliable and useful for many applications high power/energy systems, under a variety of operating conditions, pulsed, continuous wave, rep-rated or burst mode of varying duty cycles which is the most important for the wide spectrum of applications. LD array and matrix, disk laser geometry are the most important applications for this advanced technology.  Due to the efficient porous cooling technology and possibility of amplified spontaneous emission suppression the operation of the big size mono-module disk laser geometry is possible in CW and pulse-periodic modes at extremely high output energy/power. The most important historical steps of high power/energy optics development will be presented in the talk. Analysis of a wide range of materials for high power/energy optics on the basis of stability parameters has shown that silicon carbide produced either by reaction bonding or sublimation has an advantage over other materials in all well known criteria. Some new areas of SiC technologies implementation will be presented as well.  New challenges for contemporary investigations and new areas of application for high power/energy optics technology and ideas will be highlighted

Biography:

Robson Alexandre Brochetti has a degree in Pharmacy and Biochemistry from Universidade Nove de Julho, with an emphasis on Clinical and Toxicological Analysis. Specialization in Clinical and Hospital Pharmacy; Master in Biophotonics applied to Health Sciences area of concentration: Phototherapy and Dosimetry and PhD in Biophotonics applied to Health Sciences area of concentration: Phototherapy and Dosimetry. He is currently a Professor in the Pharmacy undergraduate course at Universidade Nove de Julho - UNINOVE in the areas of Clinical Pharmacy and University Pharmacy (Pharmacy School) and with a research project in the doctorate of asthma resistant to corticosteroids.

Abstract:

Timing:15:35:00 to 15:50:00 EDT 


Asthma is a chronic inflammatory disease characterized by lung cell recruitment and airway hyperresponsiveness. Treatment consists of bronchodilator antagonists, corticosteroids, or leukotrienes. Photobiomodulation (PBM) appears as a possibility in the treatment of lung diseases, and we focus on evaluating the effects of PBM on lung function in the experimental model of asthma. Male wistar rats were sensitized or challenged with ovalbumin (OVA) and treated or not with PBM (1h and 4h after each challenge with OVA). After 24 h of the last challenge with OVA, lung function and levels and gene expression of nitrites and eicosanoids were evaluated. Wavelength: 660 nm; Power: 160 mW; Power density: 38.5 mW/cm²; Energy density: 5.8 J/cm²; Exposure time: 150 s; 24J energy;The irradiation was performed at a single point, reaching the trachea and lungs. The data showed that PBM in allergic rats caused reduced respiratory resistance (Rrs), respiratory elastance (Ers) to cholinergic stimuli. The effects appear to be dependent on the balance of nitric oxide and eicosanoids since we have demonstrated high levels of nitrites concomitantly with reduced levels of eicosanoids in lung explants. We have also shown increased nitric oxide synthase (NOS) gene expression and decreased gene expression of cyclooxygenase enzymes (COX1 and COX2) in lung tissue after treatment with PBM. The data presented show that treatment with PBM reduced respiratory mechanics during the allergic response, altering the nitric oxide/eicosanoids ratio and opening the possibilities of treating episodes of bronchoconstriction.

Poster:

Biography:

Andreia La Selva is a dental surgeon, specialist in stomatology and PhD student in biophotonics applied to health sciences at Universidade Nove de Julho, São Paulo, Brazil. His line of research is in photodynamic therapy.

Abstract:

Timing: 09:50:00 to 10:05:00 EDT 

The labialis infections by Herpes Simplex Virus type 1(HSV-1) are contagious and cause discomfort and pain, in addition to being recurrent. The gold standard treatment is acyclovir, but there is viral resistance and does not prevent a recurrence. Antimicrobial photodynamic therapy (aPDT) is a promising approach because, in addition to its excellent topical antiviral effect, it does not induce resistance and prevents recurrence, according to some case reports. This controlled, randomized, double-blind, twelve-month follow-up clinical trial aims to compare the use of aPDT (660nm, 100mW, 120J/cm², 0.005% methylene blue) with topical acyclovir therapy in the treatment of herpetic lesions in stages of vesicles and ulcers through the evaluation of the time to resolve the lesion Two groups: G1- experimental group- aPDT and acyclovir placebo (n= 12) and G2- control group- acyclovir treatment and aPDT placebo (n= 12) will be formed. Secondary variables are pain (visual analogic scale), analysis of cytokines by ELISA (IL1β, IL-6, TNF-α, IL, 10), quantification of HSV-1 by RT-qPCR, and recurrence. The lesions will be evaluated 3 and 7 days after the proposed treatment (treatment effectiveness). Follow-up in month 1, 6 and 12 after intervention (recurrence assessment). A questionnaire will assess the impact of oral health on the participants' quality of life (ohip-14), on the first day and after one year of care. For statistical analysis: ANOVA two-way, complemented by the Bonferroni test. We expect a shorter interval of time for the remission of the disease, without recurrence of the lesion at the site where aPDT was applied.

Poster:

Biography:

Marlene Ferreira works at Nove de Julho University as a professor in the Health Department. She teaches Anatomy and Physiology to Health Science undergraduate students for the last 5 years. She has a Higher Education Specialization and a Master’s degree in Morphofunctional Sciences from the University of São Paulo in 2014. Her primary research interests are in the field of cellular biology and cancer. Specifically, nowadays, she is interested in oral squamous stem cells and the influence of photodynamic therapy. She is developing her doctoral thesis at Nove de Julho University in Biofotonic Applied to Health Science.

Abstract:

Timing: 15:50:00 to 16:05:00 EDT 

Introduction: Squamous cell carcinoma is the most prevalent malignant neoplasm in the oral cavity. The main therapeutic modalities of OSCC are surgery and radiotherapy alone or in combination with cisplatin. Photodynamic Therapy (PDT) is based on the use of photosensitizers, whose cytotoxicity is activated by light, leading to the death of target cells. Some studies have demonstrated that PDT can be an alternative treatment to OSCC in initial stages. Objective: The aim of this study was to evaluate the effects of PDT on the cellular viability of OSCC cell lines using different dosimetric parameters.  

Material and Methods: CA1 and Luc4 cell lines were cultivated in DMEMF12+10%FBS and supplements. 5x104 cells were plated in 96-well plates and divided into the following groups: control, LED, 5-aminolevulinic acid (5-ALA) and 5-ALA-PDT (5-ALA+LED). Cells in the 5-ALA and PDT groups were incubated with 0.5 mM; 0.75 mM e 1mM 5-ALA at 37ºC for 4h. Next, cells were washed, and the groups LED and PDT were irradiated with a diode emission light (LED) using the BioLambda LedBOX, 660nm, 3J/cm² or 6J/cm², 49.5 mW/cm² and 40% or 80% of power capacity. Cell viability was evaluated after 24h using the MTS and neutral Red assays.

Results: CA1 and Luc4 cell lines showed a significant decrease in cellular viability after 5-ALA-PDT with 6J/cm2, 5-ALA at 1mM, and 80% of the power. No difference in cell viability was observed with 3J/cm² or 6J/cm² and 40 % and 80% of power in both cell lines. In addition, a significant decrease in neutral red incorporation was noticed in PDT groups (1mM 5-ALA, 6J/cm^2, and 80% of power) from CA1 and Luc4 when compared to all groups.

Conclusion: PDT was able to decrease the cellular viability in OSCC cell lines, and further studies are needed to evaluate its effects on other biological processes in OSCC.

Poster:

Biography:

Vahideh Tahmoorian got her Ph.D. from Tabriz university and started teaching at Islamshahr Azad University as an assistant professor. She is also working at a telecom company that inspires her to work on quantum networks.

Abstract:

 Timing: 09:35:00 to 09:50:00 EDT 

Entanglement is a key feature in quantum information theory. Recently, quantum mechanics in biological systems have attracted lots of attention. A major interest is toward brain science. It is hypothesized that reality comes from the wave function collapse (Tuszynski, n.d.). So they believe that classical reality arises from brain cell's decoherence. The introduction of quantum coherence in brain cells can probably open up a new window to the brain functionality as a quantum computer. It should be noted that many quantum features are destroyed in a large, warm, wet, noisy environment. Therefore it seems that quantum effects don't survive in vivo. On the other hand, it is proved that brain cell's mitochondria can emit biophotons (Rahnama et al., 2011). Microtubules are composed of tubulin dimers to form cytoskeletons of the cell. Bose et al. show that irrespective of the temperature of the field, for a thermal state of a cavity field, the entanglement can arise. In this paper, the quantum entanglement between cellular biophotons and tubulin states is investigated. It is theoretically observed that some entanglement oscillations occur near the microtubule. We use thermal states to present and explain the oscillations. When it comes to the transfer of information in the brain, these oscillations may play an essential role.

Biography:

Markus Lücking studied engineering at the Technical University of Braunschweig and Zaragoza. He´s working as a research scientist at the FZI Research Center for Information Technology. His research interest is in the modeling of laser-tissue interactions.

Abstract:

Many experimental studies have been focusing on physical damage mechanisms of short-term exposure to laser radiation. In the nanosecond (ns) pulse range, damage in the Retinal Pigment Epithelium (RPE) is most likely to occur at threshold levels due to bubble formation at the surface of the strongly absorbing melanosome. In order to model the bubble formation threshold and, therefore, the damage threshold, the energy uptake of the melanosomes is one key aspect. In this work, a thermal finite volume model for investigations of temperature rise and temperature distribution of irradiated melanosomes is presented. The model takes different geometries of the melanosome into account as well as heterogeneous energy absorption of the melanosome. For the first time, the effect of size and shape variation on the melanosomes' thermal behavior is considered. The calculations illustrate the effect of the geometry on the maximum surface temperature of the irradiated melanosome and thus the impact on the bubble formation threshold. A comparison between the calculated bubble formation thresholds and the RPE cell damage thresholds within a pulse range of 3 to 5000 ns leads to a mean deviation of  with a standard deviation of . The best agreement between the simulation and available RPE cell damage thresholds is achieved for pulse durations close to the thermal confinement time of single melanosomes.

Biography:

Naylya Djumaeva has completed her Ph.D. at the age 40 years from Institute for Epidemiology, Microbiology and Infectious Diseases (Uzbekistan) Naylya works as the Consultant – Neurologist at the Research Institute of Virology(Uzbekistan) She is the author of 36 papers including reputed journals, and she has 2 patents.

Abstract:

This poster presents the results of the study of torsion field application in medicine Torsion fields were used with the aim to transfer information from the medicine to the patient's body. The group of patients with different chronic conditions such as chronic hepatitis B and C virus infections, patients with cytomegalovirus infection, patient with Epstein- Barra infection was treated with the application of laser light guide, where the source of light was low-level laser radiation. The patients were under observation in the Out-Patients Department of Research Institute (Uzbekistan), and the approach to therapy towards the patients was the same: torsion fields formed by the radiation of low-level laser therapy device. Into the field of radiation, the medicine was placed to be transmitted to the patient's body. Our approach to therapy has patented. The obtained results have shown that under the torsion field, formed with the help of a laser light guide, the distant transmission information from medicine to the human body occurred. The method of therapy is safe and might be used to treat patients with chronic viral diseases. In some cases, it results in the complete elimination of the virus infection (hepatitis C virus infection). In patients with a virus pathology of the liver, this therapy leads to the improvement of quality of their life and contributes to the prevention of the development of such terrible complications of chronic viral such as cirrhosis of liver and cancer of the liver

Biography:

Angela Molon graduated in Dentistry at Nove de Julho University, São Paulo, Brazil, in 2017. During her graduation, she worked as a volunteer at the Research Laboratory, focusing on Molecular and Cellular Biology, Immunology, and stem cells. Nowadays, she is a Master’s student at Nove de Julho University in the Postgraduate Program of Biophotonic Applied to Health Science. She is studying the effects of photodynamic therapy and immunotherapy in the treatment of oral squamous cell carcinoma.

Abstract:

Timing: 16:35:00 to 16:50:00 EDT 

The presence of the cancer stem cells (CSC) in oral squamous cell carcinoma (OSCC) has been previously associated with recurrence, resistance to conventional therapy, and poor prognosis. Thus, there is an urgent need to improve the treatment of OSCC, mainly for patients with recurrent or metastatic disease. Photodynamic therapy (PDT) is a minimally invasive therapy able to promote cell death directly, immune surveillance, and disrupts tumor vasculature. Thus, this study evaluated the subpopulations of CSC identified with the phenotype CD44^high/ESA^high and CD44^high/ESA^low in OSCC cell lines as well as the expression of MICA/B (stressed-induced ligand for Natural Killer cells). Ca1 and Luc4 cell lines were divided into control, LED, 5-aminolevulinic acid (5-ALA, 1mM), and 5-ALA-PDT (5-ALA+LED) groups. Cells were irradiated with a diode emission light (LED) using the Bio Lambda Led BOX, 660nm, 6J/cm², 49.5 mW/cm². Cells were incubated for 24h and then collected to evaluate the expression of CD44, ESA, and MICA/B by flow cytometry. The subpopulation of CSC with the phenotype CD44^high/ESA^high (epithelial morphology) was significantly decreased after PDT when compared to control, LED, and 5-ALA groups. However, no difference was observed in the percentage of cells with the phenotype CD44^high/ESA^low (mesenchymal morphology)in all groups. MICA/B expression was induced after PDT. In conclusion, PDT was able to decrease the CD44^high/ESA^high cells and to promote the expression of MICA/B, contributing to eradicate the CSC and probably, to the activation of immune cells.

Poster:

Biography:

EDUARDO VASQUES DA FONSECA is a Ph.D. student of Post graduation Program in Biophotonics Applied to Health Sciences, Nove de Julho University – UNINOVE, São Paulo, Brazil, Specialist in Surgery and Traumatology Oral Maxillofacial; Coordinator of the Buco Maxillofacial Surgery and Traumatology Service of the Mandaqui Hospital Complex and at Hospital Dr Osirís Florindo Coelho, São Paulo, Brazil.

Abstract:

Timing: 14:20:00 to 14:35:00 EDT 


The proposed study aims to evaluate the efficacy of LED devices regarding the control of pain, edema, paresthesia, and bone repair following surgical maxillary disjunction. The secondary aims are to evaluate the degree of anxiety and the impact of the surgical procedure on quality of life. A randomized, controlled, double-blind clinical trial will be conducted with 72 patients. The procedures will be performed by Three surgeons. Two examiners blinded to the allocation of the patients to the different experimental groups will perform the preoperative and postoperative evaluations, and another researcher will administer PBM. Prior to each surgery, five facial measurements, a periapical radiographic exam, facial and oral sensitivity tests, and evaluation of anxiety (Beck questionnaire and determination of salivary IL-1β, IL-6, TNF-α, and cortisol) will be performed. Immediately after the surgeries, the participants will be randomly allocated to the active and sham PBM groups. In the active group, the participants will receive nine applications of PBM (immediate postoperative period, 1-120 days) with a facial device (57 LEDs at 660 nm and 74 at 850 nm, 5 mW; 6J per point) and an intraoral device (3 LEDs at 660 nm, 5 mW; 2J per point). In the sham group, irradiation will be simulated. Data will be collected in periods up to 120 days after surgery, depending on the variable analyzed. A normality test will be used to determine the appropriate statistical tests for each dataset, with the level of significance set to 5%.

Poster:

Biography:

Adriana Mazzoni is a master's student in the Biophotonic' s Program applied to Health Sciences at Universidade Nove de Julho in São Paulo, Brazil. She has been a specialist in pediatric dentistry at the University of Santo Amaro in São Paulo since 1994, has been a Trainer in the Evaluation of the tongue brake by the Ministry of Health of Brazil since 2018, has been a teacher at the Essekabe School of Dentistry in São Paulo since 2018. She studies and works with breastfeeding since 1995, and is very interested in studying in detail the whole subject related to this theme.

Abstract:

Timing: 11:35:00 to 11:50:00 EDT 

Ankyloglossia is an anomaly that is characterized by an abnormally short or thick lingual frenulum that can restrict the movements of the tongue and has been identified as one of the factors that can negatively interfere with breastfeeding, decreasing the ability of the newborn to handle proper. The repositioning of the lingual frenulum, also called lingual frenotomy, is the most recommended for infants because it is a less invasive technique. This surgery can be performed conventionally with a scalpel blade or with thermal resources such as an electric scalpel or high-powered lasers.
Participants in this study will be infants between 0 to 3 months of age diagnosed with ankyloglossia, difficulties during breastfeeding, need for lingual frenotomy and will be randomly distributed into groups and divided into groups: Surgery group with electrocautery and Laser surgery group high power diode.

The objective of the study will be to evaluate the release of the lingual frenulum through the lingual frenotomy performed with a high power diode laser or electrocautery techniques.

Poster:

Biography:

Rafael Zaratin Beltramin is a dental surgeon, a master's degree student in Rehabilitation Sciences from Universidade Nove de Julho, a specialist in Public Health from Universidade Nove de Julho and graduated in Dentistry from Universidade Nove de Julho. Rafael Zaratin Beltramin has improvement and complementary courses in the areas of bruxism, minor oral surgery, dental aesthetics, botulinum toxin, and facial fillers.

Abstract:

Timing: 16:05:00 to 16:20:00 EDT 

The aim of this systematic review will be to analyze whether children with cerebral palsy (CP) have abnormal bilateral masseter and temporal muscle activation during mastication. This review will follow the recommendations of the Cochrane Handbook for Systematic Reviews of Interventions and PRISMA statement and will be registered at the PROSPERO platform. A comprehensive search of the literature will be performed using an electronic search (MEDLINE, Embase, Cochrane Library, LILACS, BBO, Clinicaltrials.gov, and WHO/ICTRP) with no restriction regarding date and language. The grey literature will also be screened via OpenGrey. We will include randomized (RCT) comparative observational studies that assessed as primary outcome masticatory muscles (temporal and masseter) activation, by using electromyography parameters such as time of activation, duration, masticatory cycle, and force peak. Two authors will independently select the references retrieved by search strategy using the software Rayyan and will extract the data from included studies. All discordance will be solved by a third author. The risk of bias assessment will be performed using the Newcastle-Ottawa scale. We will calculate the mean differences for continuous outcomes and risk ratios for dichotomous outcomes (CI 95%). If possible, electromyography parameters will be combined in a meta-analysis using the random-effect model in the Review Manager 5.4 software. Heterogeneity between studies will be evaluated. The certainty of the body of the evidence will be assessed using the GRADE approach.

Biography:

Erick Frank Bragato is Ph.D. student of the Postgraduation Program in Biophotonics Applied to Health Sciences, Nove de Julho University – UNINOVE, São Paulo, Brazil. Graduated in medicine in 2014 from the State University of Londrina, Brazil, has a medical residency in General Surgery and is completing his residency in Plastic Surgery in São Paulo, Brazil.

Abstract:

Timing: 10:35:00 to 10:50:00 EDT 

Skin aging is an irreversible, slow, and progressive process, being mainly influenced by age, but also by external factors, such as ultraviolet radiation, smoking, alcohol, among others. Studies have shown the benefits of photobiomodulation (PBM) for facial rejuvenation, especially with the use of the red LED. However, there is still a high level of variability in the treatment parameters and the frequency of application of FBM. The purpose of this study is to compare the effects of PBM with LED mask (660nm, 6.4 mW/cm², 2.67 J/cm²) on facial rejuvenation using 2 application frequencies: one group will receive 2 applications a week for 4 weeks, and the other group will receive 3 applications a week for the same period. After 30 and 90 days, the depth and length of the wrinkles (evaluation of facial impressions by optical coherence tomography); the viscoelasticity of the skin; the evaluations of photographic images by experts (Wrinkle Assessment Scale) and the level of satisfaction of the participants (FACE-Q) will be compared with the data collected before the beginning of the study. All data will be analyzed statistically according to their distribution, seeking a level of statistical significance of 5%.

Poster:

Biography:

Monise Mendes Rocha is a dental surgeon, a master's degree student in Rehabilitation Sciences from Universidade Nove de Julho, a specialist in Public Health from Universidade Nove de Julho, and graduated in Dentistry from Universidade Nove de Julho. Monise Mendes Rocha has improvement and complementary courses in the areas of bruxism, minor oral surgery, dental aesthetics, botulinum toxin, and facial fillers.

Abstract:

Timing: 16:20:00 to 16:35:00 EDT 

The aims of this systematic review will be to evaluate the effects (benefits and harms) of photobiomodulation for treating masticatory muscle spasticity in children with cerebral palsy. This review will follow the recommendations of the Cochrane Handbook for Systematic Reviews of Interventions and PRISMA statement and will be registered at the PROSPERO platform. A comprehensive search of the literature will be performed using an electronic search (MEDLINE, Embase, Cochrane Library, LILACS, BBO, Clinicaltrials.gov, and WHO/ICTRP) with no restriction regarding date and language. The grey literature will also be screened via OpenGrey. We will consider randomized (RCT) and non-randomized clinical trials (NRCT), that assessed the use of photobiomodulation compared with placebo, no intervention, or another active intervention. Primary outcomes will be muscle spasticity relief, pain intensity, and adverse events. Secondary outcomes will be muscle fatigue, the amplitude of mouth opening, and tooth wear prevention. Two authors will independently select the references retrieved by search strategy using the software Rayyan and will extract the data from included studies. All discordance will be solved by a third author. The risk of bias assessment will be performed using the RoB tool for RCTs and ROBINS-I for NRCT. For the treatment effects estimative, we will calculate mean differences for continuous outcomes and risk ratios for dichotomous outcomes (CI 95%). When possible, treatment effects will be combined in a meta-analysis using the random-effect model in the Review Manager 5.4 software. Heterogeneity between studies will be explored. The certainty of the evidence will be assessed using the GRADE approach.

Biography:

Felipe Murakami Malaquias da Silva graduated in Dentistry from the University of Dentistry, University of São Paulo (FOUSP). Specialist in Orthodontics, Ortogeo - Faculdade Sete Lagoas / São José dos Campos, SP - Brazil. Master and Ph.D. student in Biophotonics Applied to Health Sciences - University Nove de Julho. Presented works at congresses, including at WALT-2018 (Nice, France). His line of research focuses on the use of lasers and their cellular repercussions.

Abstract:

Timing: 10:20:00 to 10:35:00 EDT 

With the loss of teeth, the adjacent tooth moves towards the space left, causes aesthetic and functional problems. The skeletal anchoring system, as mini-implant (MI), has been increasingly used to replace the adjacent tooth to the correct place. Some photobiomodulation (PBM) protocols have been shown to accelerate orthodontic movements, with analgesia and inflammatory modulation. This study will evaluate how PBM interferes with molars verticalization movement, pain, and periodontium's inflammation. A selection of 34 patients with an inclined molar were randomly divided into 2 groups: G1 - verticalization with MI + PBM simulation; G2 - verticalization with MI + PBM. The PBM protocol will be: power of 100 mW, λ 808 nm, 1J per point, 10 points, for 10s, radiant exposure of 25 J / cm². Each 30 days, an orthodontic force will be applied, and PBM will be done at 0, 3, and 7 days, every month, for 3 months. By ELISA, the cytokines IL1β, IL-6, IL-8, IL-10, and TNF-α from the crevicular gingival fluid will be analyzed. We will use an initial and final panoramic radiography to measure the tilt speed. A Visual Analogue Scale and a count of painkillers will be used to assess the laser's analog effect. OHIP-14 questionnaire will be applied to identify the impact of oral health on the participant's quality of life. The ANOVA-one way test will be applied, with a mean ± SD and p-value <0.05. We expected an increase at inclination speed, a decrease in the amount of inflammatory cytokines, and an increase in anti-inflammatory.

Poster:

Biography:

Juliana Terra Fernandes is a master's student in "Biofôtonia applied to health sciences" at Nove De Julho University. Graduated in Dentistry in 2001, from the University of Mogi Das Cruzes. Specialist in Public Health, at São Leopoldo Mandic College, Specialist in preceptor ship at Hospital Sírio Libanês. She has been working in basic public health for 17 years. She acts as a dental surgeon in the city of Mogi das Cruzes. Her goal is to bring new ideas and technologies to the area of dentistry, focused mainly on public health.

Abstract:

Timing: 10:50:00 to 11:05:00 EDT 

Dental Caries disease is controlled through biofilm control, which balances demineralization and remineralization reactions, considering individual factors to prevent a recurrence. However, there are still few studies that make it possible to systematize a new protocol for the evaluation of dental biofilm through the use of light for the implantation of new biofilm control protocols through the use of optical fluorescence diagnosis. Fluorescence appears clearly in the plaque in red tones, showing the presence of microorganisms in regions where there is biofilm accumulation. 

Objectives: To evaluate the applicability and effectiveness of the diagnosis of oral biofilm with the optical fluorescence technique using the EVINCE (LED with emission centered at 400 ± 10nm - MMOptics, Brazil) equipment and to create a clinical evaluation protocol for the presence of biofilm using the same equipment. Furthermore, we intend to compare the efficacy of the optical fluorescence diagnostic method with the traditional method (Fuchsin-based dye biofilm) and to observe whether EVINCE alone would be sufficient for the evaluation of dental biofilm in young people with mixed dentition without use of traditional dyes. 

Methods: Children (6 to 12 years) will be evaluated by 3 different professionals, starting the evaluation where the 1st evaluator will note the IHOS observing only with EVINCE. In the second stage, a 2nd evaluator will perform the traditional disclosure technique with Fucsina, later noting the IHOS, and finally, 3rd evaluators who will observe with EVINCE the teeth previously stained in stage 2. We expect to find advantages with EVINCE. 

Acknowledgement: This study was financed in party by the “Coordenação de aperfeiçoamento de pessoal de Nível superior-Brasil (Capes) –Finance Code 001”

Poster:

Biography:

Marina Bertoni Guerra is a doctorate student in Biophotonics Program applied to Health Sciences at Nove de Julho University, São Paulo, Brazil. She is a gynecologist and obstetrician, graduated from the Hospital de Clínicas de Porto Alegre and her subspecialty is in the area of Fetal Medicine. She currently works as a gynecologist and obstetrician in the city of São Paulo. Her primary research interests are in the field of women’s health.

Abstract:

Timing: 11:05:00 to 11:20:00 EDT 

Menopause is a physiological event and is defined as the loss of ovarian follicular activity, with the consequent permanent cessation of menstrual cycles. Its diagnosis is made retrospectively after 12 months of amenorrhea, with no pathological cause involved. This period is marked by several changes in the female organism, mainly the genitourinary syndrome (GUS), which is a collection of signs and symptoms resulting from the state of hypoestrogenism. Almost half of the postmenopausal women will experience symptoms of GUS, with vaginal dryness being the most prevalent, followed by dyspareunia and vulvovaginal irritation. The aim of the study is to evaluate the effect of photobiomodulation with red LED (light-emitting diode) on the symptoms of vulvovaginal atrophy in postmenopausal women. A randomized and controlled clinical trial will be developed, which will include postmenopausal women with signs and symptoms of vulvovaginal atrophy. Participants will be allocated into two groups: those who will undergo photobiomodulation with intravaginal LED, and those who will receive vaginal cream with estriol. Objective and subjective improvement of atrophy will be assessed using the Vaginal Health Index and the visual analog scale, respectively. Vaginal pH, cell maturation index, and changes in sexual function through the Female Sexual Function Index questionnaire will also be assessed. The variables will be analyzed at the time of admission, in one and three months after the intervention.

Poster:

Biography:

Tânia Oppido Schalch is a dentist, specialist in periodontology and joined, in 2019, the Nove de Julho University as a Master´s student in Biophotonics applied to health sciences program, collaborating in the development of many projects. Attended college at São Paulo University (USP), where she conducted, for 5 years, research in the area of oral microbiology. Tânia has knowledge about laser therapy, microbiology, periodontology, and has experience with clinical and laboratory research.

Abstract:

Timing:10:05:00 to 10:20:00 EDT 

Pericoronitis is a prevalent condition during the eruption of third molars, many times debilitating. The most indicated treatment (initial phase) is the irrigation. There is no adequate treatment at this phase, and evolution of disease may require antibiotic therapy. To reduce systemic dissemination of infection and antibiotics use, it is important to test the efficiency of treatments in the initial phase of pericoronitis. Antimicrobial photodynamic therapy (aPDT) is an interesting alternative because it is easy to perform and does not cause bacterial resistance. The aim of this study is to evaluate the effectiveness of aPDT in pericoronitis in its initial phase. In this controlled clinical trial, 34 healthy young individuals with pericoronitis will be randomized into the positive control group  (n = 17): irrigation with sterile saline and aPDT (methylene blue-MB, 0.005%, λ = 660 nm ^, 9J per point,  318 J/cm²) and experimental group (n = 17): treatment identical to G1, however, MB will be delivered in a new formulation for oral use. Microbiological quantification will be performed for Tannarella forsythia by PCRq. Saliva and gingival crevicular fluid will be collected to evaluate cytokines. Also, it will be analyzed the pain, edema, and buccal opening and the impact of oral health on quality of life. The variables will be evaluated in the baseline and 4th day after aPDT. For statistical analysis: ANOVA two-way, complemented by the Bonferroni test. We expect that aPDT will improve clinical parameters and reduce the amount of T.forsythia.

Poster:

Biography:

Alexandre Morais has been a university professor at UNINOVE for 4 years, but he has been teaching for over 14 years. He finished his degree in Dentistry in 1997, obtained his Master's degree in 2010, and is a Ph.D. student at UNINOVE. He is also an international consultant and speaker for a dental products company based in Germany. He has been in clinical practice since 1997 with an emphasis on cosmetic dentistry.

Abstract:

Timing: 14:50:00 to 15:05:00 EDT 

With the increase in aesthetic requirements in Dentistry, alternatives were sought for the elimination of metallic structures. Ceramics are the materials of choice for dental prosthetic rehabilitation because they have many enviable properties. Among them are: aesthetics, abrasion resistance, biocompatibility, little plaque accumulation, low thermal conductivity, and color stability. Zirconia structures are widely used. However, the great disadvantage of zirconia is the low potential for adhesion to resin cement. Unlike conventional porcelains where the vitreous phase can be conditioned by hydrofluoric acid, creating mechanical retentions on the internal surface of the piece, zirconia has a high crystalline content without any vitreous phase at the edges of the 'crystalline organs.' Thus, it is inert to acid conditioning, and its wettability with the cementing (bonding) agent is impaired. In an attempt to increase the mechanical retention between zirconia and cement, some treatments are suggested by the literature: Sandblasting with aluminum oxide and high power lasers. Although there is much information about the effects of this irradiation on enamel and dentin, little is known about the irradiation of this laser as a surface treatment for high-strength dental ceramics. The aim of this work will be to compare the surface roughness and morphological characteristics of Zirconia ceramic surfaces irradiated with Er: YAG laser with the variation of radiant energy.

Poster:

Biography:

Andréia Martinelli de Siqueira Araujo was graduated in physical therapy by the Nove de Julho University. During the undergraduate period, she participated in a scientific initiation project, which allowed her to know the research better. After she obtained her Master's Degree in Rehabilitation Sciences Post Graduate Program at Nove de Julho University, and she is currently a Ph.D. student in the same Post Graduate program. The main objective of her research area is to evaluate the plasticity of the skeletal muscle.

Abstract:

Timing: 11:50:00 to 12:05:00 EDT 

Skeletal muscle is a tissue with high adaptive capacity altering its characteristics to meet its diverse functional demands. Compensatory hypertrophy (CH) occurs due to excessive mechanical load on a muscle, promoting an increase in the size of muscle fibers. Photobiomodulation (PBM) has demonstrated beneficial effects on muscle tissue during CH. This study aimed to evaluate the effect of PBM on the inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) during the process of CH. Wistar rats were divided into three groups: control group (n=5), hypertrophy (H) group (n = 10) and Hypertrophy + PBM group (n = 10). CH was induced through the ablation of synergist muscles of the plantaris muscle. The preserved plantaris muscle below the removed muscles was submitted to excessive functional load. PBM was performed with low-level laser (AsGaAl, λ = 780 nm; 40 mW; energy density:10 J/cm²; 10 seconds on each point, 8 points; 3.2 J). Animals were euthanized after seven and 14 days. The plantaris muscles were removed and sent for analysis of the gene expression of IL-6 and TNF-α using qPCR. The gene expression results demonstrated an increase in IL-6 in the H+PBM group in comparison to the H group at 14 days as well as an increase in TNF-α in the H+PBM group in comparison to the H group at seven days. Based on findings in the present study, it is concluded that PBM was able to modulate pro-inflammatory cytokines that are essential for the compensatory hypertrophy process.

Poster:

Biography:

Eloiza Helena Brandão Calheiros has graduated at the Dentistry school of São José dos Campos-UNESP. She undertook scientific initiation in the department of basic sciences, in the area of Microbiology. Realized a Multi professional residency in a Family Healthy Programa. Completed a specialization in Periodontics by the  São Paulo Association of Dental Surgeons-APCD. Also, realized a specialization in Implantology by FUNDECTO-USP. Master´s student in the field of Biophotonics at  Nove de Julho University.

Abstract:

Timing: 12:50:00 to 13:05:00 EDT 

Oral Lichen Planus (OLP) is a chronic mucocutaneous inflammatory disease with different clinical presentations, degrees of discomfort and pain. Deregulated release of cytokines have been identified associated with its pathogenesis. The standard treatment for OLP patients is topical or systemic corticosteroids, which can lead to side effects and/or a lack of response to therapy. The Photobiomodulation (PBM) is an effective treatment in inflammatory diseases, modulating inflammatory cytokines, promoting analgesia and tissue repair. The aim of this study was to evaluate the serum concentration of TNF-α and IL-17 in OLP patients treated with PBM. Thirty-four patients with OLP were randomized into the following groups: Control (topical 0.05% clobetasol propionate, 3 times a day for 4 weeks) and PBM (laser twice a week, 680 ± 20nm, 100mW, 177J/cm², 5s, 0.5J, for 4 consecutive weeks).Peripheral blood was collected at baseline (D0) and at the end of treatment (D30) and the serum levels of TNF-α and IL-17A were evaluated by ELISA. The results were then correlated with pain and OLP clinical presentation. In PBM and control groups, higher levels of serum IL-17A was observed at baseline when compared to TNF-α (p<0.05). No significant difference in the concentration of TNF-α and IL-17A was found at D30 when compared to baseline in both groups. In addition, no correlation between the serum levels of TNF-α or IL-17A with pain and clinical OLP presentation was observed. In this study, no modulation of the inflammatory cytokines TNF-α or IL-17A was found after treatment with PBM or corticosteroids.

Poster:

Biography:

Erika da Silva Mello is a graduate student in Biophotonics at Universidade Nove de Julho. She had a major in Dentistry at Universidade Paulista in 1993, she dedicated herself to general practice with an emphasis on orofacial pain and temporomandibular joint disorders, having completed this specialty in 2009 at Universidade São Marcos. She is also a specialist in acupuncture by the Escola de Terapias Orientales de São Paulo in 2012 and qualified in the clinical use of laser by the University of São Paulo (2017) in the dental clinic, includes training in laser high power and experience in functional rehabilitation. Her main interest is in the field of research in Biophotonics associated with the patient's quality of life.

Abstract:

Timing: 12:35:00 to 12:50:00 EDT 

Preconditioning the tissue offers good results in preventing mucositis in cancer treatments and in preventing muscle injuries in fatigue protocols. On the other hand, in third molar surgeries, photobiomodulation has been widely used in the treatment of postoperative complications such as pain, edema, and trismus. This study aims to evaluate the effects of photobiomodulation preconditioning in third molar surgeries. This is a clinical, randomized, double-blind, and placebo-controlled study. Adults over 18 years old, with an indication of removal of third molars, compatible with the inclusion criteria, after signing the Informed Consent Term, their facial and interincisal measurements were measured. They received treatment according to randomization (treatment group-TG / control group-CG), and after one hour, they underwent removal of the third molar. After 48h and 7 days, measurements were taken, pain analysis according to a visual analog scale and randomized treatment. At the conference, we will present the preliminary results of this clinical trial.

Poster:

Biography:

Frederico Carlos Jana Neto is a physician (USP, Brazil). Medical residency in Orthopedics and Traumatology and Fellowship in Hip and Knee Replacements (IOT HCFMUSP). MBA in Health Management (Insper). Ph.D. student in Biophotonics and Professor at Nove de Julho University. Preceptor of medical residency in orthopedics and traumatology at Mandaqui Hospital Complex, SP, Brazil. Head of the Knee Arthroplasty Group at the Prevent Senior Institute.

Abstract:

Timing: 14:35:00 to 14:50:00 EDT 

Photobiomodulation (FBM) is indicated as an adjuvant treatment to accelerate wound healing, however, there is still a lack of evidence regarding its effect on traumatic soft tissue injuries. This project aims to evaluate the effects of FBM in the resolution of complex soft tissue injuries of traumatic origin associated with tibial fractures. 84 adult individuals, aged between 18 and 60 years, hospitalized with tibial fractures awaiting resolution of soft tissue injuries will be included to undergo definitive surgery. The subjects will be randomized in two groups: FBM (treated with a device with 144 LED emitting diodes at wavelengths of 420, 660 and 850nm, 3J per point for 10 minutes) and Sham (simulation of the LED application, with a device with characteristics identical to that of the FBM group, for the same period of time). Subjects will be treated daily until release for surgery. The primary outcome will be the assessment of the wound healing process using the Bates-Jensen scale. Secondary outcomes will be: pain intensity, consumption of analgesic drugs, serum evolution of inflammatory markers C-reactive protein and creatine kinase, measurement of the lesion area, the time needed for release for definitive surgery, presence of infection, and the effective effectiveness of FBM. The evaluations will be carried out before the beginning of the intervention and daily until the participant is considered ready for surgery (which will be considered the end of the experimental period). The data will be analyzed statistically, considering a significance level of 5%.

Poster:

Biography:

Juliana Hironaka is a dentist with graduation and specialization in endodontics at Methodist University in Sao Paulo, Brazil. She received a degree of specialist in microscopy and endodontic microsurgery at the Association of Dental Surgeons of Sao Paulo in 2010.  With great interest in the field of biophotonics she started a research project at the University Nove de Julho relating endodontics and respiratory diseases in 2019. At the present time she works in the area of endodontics in a private clinic. Her future project involves teaching and researching in this field.

Abstract:

Timing: 13:35:00 to 13:50:00 EDT 

Apical periodontitis is caused by pulpal necrosis, with consequent inflammation and destruction of the periapical tissues. It has been shown that periodontal disease can influence the symptoms of Asthma. Asthma is a chronic inflammatory disease characterized by lower airway hyper responsibility and variable airflow limitation. To date, there are few papers correlating the symptoms of Asthma in patients with apical periodontitis (AP).This study aims to evaluate the effect of photodynamic therapy with endodontic treatment on pulmonary inflammation in an experimental model of Asthma. Forty-eight male Balb/c mice were divided into 6 groups (n = 8). Basal, AP, AP + PDT, Asthma, Asthma + AP, Asthma + AP + PDT. Apical periodontitis was induced with the coronary opening, and the tooth remained open for 21 days. Asthma was induced by ovalbumin (OVA) and aluminum hydroxide, subcutaneously (days 0 and 14) challenge via tracheal oro, three times a week for six weeks. The treatment of PA was performed with calcium hydroxide and PDT with methylene blue (0.005%) associated with red diode laser irradiated 660 nm, with the energy of 287 J / cm², with 9 J at the point, delivered in 90 s. Total and differential counts of alveolar bronchial lavage (BAL) and serum alkaline phosphatase were analyzed after euthanasia. We observed an increase of lymphocytes and eosinophils of BAL in the Asthma+AP group when compared to the Asthma group. On the other hand, we observed a reduction of this cell recovered from the BAL in the Asthma+AP+PDT group about the Asthma+AP group. In addition, there was an increase in bone resorption (alkaline phosphatase) in the AP and Asthma+AP groups, but with the treatments, only the non-asthma groups reduced this parameter. It is concluded that apical periodontitis may increase pulmonary inflammation. The association of Asthma with apical periodontitis worsens the parameters of pulmonary inflammation. However, after the standard treatment with PDT of apical periodontitis, pulmonary inflammation decreased in the asthmatic mice, reaching near the basal levels.

Poster:

Biography:

Luciana graduated in Dentistry in the University of Santo Amaro (1999), a specialization in Periodontics from Paulista University (2004) and in Implantology in the Paulista Association of Dental Surgeons (2008). In 2019 she specialized in Training Teachers for Higher Education in the Nove de Julho University. She joined the master's degree in Biphotonic Applied to Health Sciences at the same institution, where she has a research project in the field of antimicrobial phototherapy in periodontics. She currently works as a specialist in periodontics and implantology in her private clinic and in the future, intends to teach, and continue researching in her area of expertise.

Abstract:

Timing:13:20:00 to 13:35:00 EDT

Periodontal disease (PD) is a chronic inflammatory disease caused by bacterial biofilm which is highly prevalent worldwide. Antimicrobial photodynamic therapy (aPDT) has been used as a complement to the conventional treatment and aims at the production of reactive oxygen species (ROS) using light and an exogenous photosensitizer to eliminate microorganisms. On the other hand, antimicrobial blue light therapy (aBLT) takes advantage of endogenous photosensitizers (such as protoporphyrin and flavin). The objective of the present study is to evaluate the potential for the bacterial kill by aBLT and the influence of the culture medium inthe death rate of Aggregatibacter actninomycetencomitans and Porphyromonas gingivalis. The protocol used will be the use of a 403nm ± 15 LED (blue / violet) with a power of 1W, the irradiance of 588, 2 mW / cm², for 0, 1, 5 and 10 min of irradiation exposure. Four groups are being studied: A. actinomycetencomitans cultivated in BHI; P. gingivalis grew in BHI; A. actinomycetencomitans grown in blood agar, and P. gingivalis grown in blood agar. The plates will be incubated in microaerophy and anaerobiosis, in a bacteriological greenhouse, with a temperature regulated at 37o C during a period of 48h to count the colony-forming units (CFU / mL) and performed in triplicate. The comet test will be carried out on the culture of microorganisms after irradiation to assess the possible DNA damage generated by antimicrobial therapy with the blue light. At the conference, we will present the first results of this work.

Poster:

Biography:

Mara Lúcia Gonçalves Diogo Graduated in Nursing from the Nursing School WenceslauBraz (1986). Postgraduate in Occupational Nursing from the Catholic University of Santos (1989), the title of Specialist in Dermatology by the Brazilian Association of Nursing in Dermatology - SOBENDE (2007). He is currently a freelance professional, working in private practice. Experience in Nursing, with emphasis on Nursing in Dermatology and aesthetics. Invited professor in specialization courses in Dermatology throughout Brazil, done in person, or through online classes. Master's student in Biophotonics at Universidade Nove de Julho, São Paulo.

Abstract:

Timing: 13:05:00 to 13:20:00 EDT 

Light therapies have been used in dermatoses, including acne. Some studies indicate that the blue LED inhibits the proliferation of C. bacterium acnes and that the red light acts to decrease the inflammatory condition. Several studies have been analyzed, some in vitro, others in vivo; however, few studies have compared and analyzed the in vivo use of LED in acne. Therefore, the objective of this study is to compare the effect of the blue LED, the red LED, and the combined red and blue LEDs in patients with mild and moderate inflammatory acne (grades II and III). 108 participants with inflammatory acne will be selected according to the classification of the Brazilian Society of Dermatology and IGA (Investigator’s Global Severity Assessment) and also the degree of skin color according to the Fitzpatrick scale (I to IV). Participants will be treated with an LED mask, applied over the entire face, for 10 minutes, 3 times a week, in the UNINOVE laboratory, divided into 3 groups, where the first group will use a mask with blue LED, the second with LED red and the third with combined blue and red LED. The evaluations will be made through photos and counts of papules and pustules, in the baseline, on the 15th and 30th day of treatment by a blind evaluator. Participants will also assess their perception of improved appearance after the end of treatment, using the CADI questionnaire (CARDIFF ACNE DISABILITY INDEX).

Poster:

Biography:

Paula Tatiane Alonso is currently pursuing a master's degree in the Post Graduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE-São Paulo-Brazil- since 2019). Physical Educator (UNICAMP- Campinas-Brasil- 2005) with Specialization in Physiology, Nutrition, Biochemistry, and Training (UNICAMP- Campinas-Brasil -2006), Specialization in Exercise Physiology (UNICAMP- Campinas-Brasil -2006), Specialization in Acupuncture (EBRAMEC-São Paulo-Brasil-2017) and Advanced Studies in Acupuncture and Moxibustion (TCM University of Shandong -China-2018). Currently teaches acupuncture photo course for students of Traditional Chinese Medicine. She is dedicated to the study of Photobiomodulation in respiratory diseases (especially asthma) and also pain and pathologies and syndromes in triathletes (and athletes in general).

Abstract:

Timing: 12:20:00 to 12:35:00 EDT 

Sepsis is a severe disease with high mortality index. Acute lung injury (ALI), characterized by alveolar damage, lung inflammation, and impaired gas exchange, is a serious manifestation of sepsis, which is not efficiently treated by current drugs. ALI is pathology with high morbidity and mortality. Photobiomodulation (PBM) has been showing good results for several inflammatory diseases. The aim of this study was to analyze the effect of systemic PBM with a red LED in the management of inflammatory parameters. Male rats were injected with lipopolysaccharide (LPS) or saline (i.p.) and, irradiated or not with light-emitting diode in the caudal vein, for 150 s, 2 and 6 h after the LPS injections. After 24 h the LPS or saline injections, the inflammatory parameters were investigated. Device specifications: Bio Lambda LEDstar, Black Box Mini Model, São Paulo, Brazil; Probe Design, Single Probe; Wavelength: 660 nm; Radiant Power: 160 mW; Power Density: 38,5 mW/cm²; spot area: 4,15 cm²; Density of energy: 5,8 J/cm²; Issuance: Continuous (cw); Total Radiant Emission: 24 J. Our results point to the beneficial effects of systemic PBM on the LPS-induced ALI, as it reduced the number of neutrophils recruited into BAL, myeloperoxidase activity and also reduced IL-1β, IL- 6 and IL-17 in the lung. Our data showed beneficial effects of transcutaneous systemic PBM treatment on ALI caused by sepsis, and suggest that PBM application as an inexpensive and non-invasive additional treatment to sepsis.

Poster:

Biography:

Stephanie Souza Soares is a dentist, graduated from UNINOVE-2012) with Specialization in Endodontic from São Leopoldo Mandic - SLM. She obtained the Master's degree in 2018, and has done Ph.D. in the Post Graduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE since 2018). She has been a university professor since 2017.

Abstract:

Timing: 12:05:00 to 12:20:00 EDT 

Chronic obstructive pulmonary disease (COPD) is characterized by chronic inflammation and alveolar enlargement. Several experimental models have been proposed for the discovery of new therapeutic options. Thus, mesenchymal stem cells (MSCs) and photobiomodulation (PBM) have been studied for their immune system modulating functions. The aim of this study was to investigate the effects of PBM associated with deciduous tooth MSCs on inflammatory mediators in bronchial epithelial cells (BEAS) induced by cigarette smoke extract (CSE). Human bronchial epithelial cells (BEAS-2B) were cultured (5x10⁴ cells / well) and after 24 hours, the cells were incubated with CSE. 1 hour later irradiated with 808nm, 30mW, 60 seconds / well diode laser and / or MSCs extracted from tooth pulp, cultivated and characterized by the presence of CD90⁺, CD73^+, and absence of CD34. After 24 hours, the supernatant was collected and the mediators were dosed. The effects of the association of PBM with MSCs on the release of cytokines (IL-6, IL-10 and IFN-g) were evaluated by ELISA.

Results: CSE increased levels of IFN-g (p<0.001), decreased levels of IL-10 (p<0.001), did not change levels of cytokines of IL-6. Groups treated with MSCs showed an increase compared to the other groups (p<0.001). There was a significant decrease in IFN-g (p<0.001), and an increase in the IL-10 (p <0.001) in the treated groups. In vitro treatment with MSCs and PBMs of CSE treated cells can be promising on the effects of the 808nm laser in the treatment of patients with COPD.

Poster:

Biography:

Simone Klein Martins is a dentist, graduated from FOP-UNICAMP- 1995) with Specialization in Endodontic. She obtained the Master's degree in 2019, and has done Ph.D. in the Post Graduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE since 2019). She has been a university professor at UNINOVE since 2016.

Abstract:

Timing: 13:50:00 to 14:05:00 EDT 

Introduction: Since asthma is a multifactorial disease whose treatment sometimes is not effective, new therapies that improve the respiratory discomfort of patients are of great importance. Photobiomodulation (PBM) has emerged as an important toll to treat lung diseases characterized by inflammation. So our objective was to investigate the effects of PBM on allergic lung disease by an evaluation of lung cell migration, mucus secretion, tracheal responsiveness, and mast cell degranulation.

Methods: Male Balb/c mice were or not sensitized and challenged with ovalbumin (OVA) and treated or not with PBM (1h and 4 h after each OVA challenge). Twenty-four hours after the last OVA challenge the analysis were performed. Analysis was performed 24h the last LED treatment or OVA challenge. Device specifications: Bio Lambda LEDstar, Black Box Mini Model, São Paulo, Brazil; Probe Design, Single Probe; Wavelength: 660 nm; Radiant Power: 160 mW; Power Density: 38,5 mW/cm²; spot area: 4,15 cm²; Density of energy: 5,8 J/cm²; Issuance: Continuous (cw); Total Radiant Emission: 24 J.

Results: Our results showed that PBM treatment in asthmatic mice reduced the lung cell infiltration, the mucus production, the tracheal’s contractile response. The effects of PBM treatment on these parameters may be modulated by mast cells, since the degranulation was decreased (P<0.05).

Conclusions: This study may provide important information about the effects of PBM, and in addition, it may open the possibility of a new approach for the treatment of asthma.

Financial support: CNPq 305099/2017-5.

Poster:

Biography:

Cristiano Rodrigo de Alvarenga Nascimento graduated in medicine at the federal university of Juiz de Fora, the same college where he completed his medical residency in Pulmonology. Specialist in Pulmonology and intensive care and with an MBA from Fundação Instituto de Administração, Universidade de São Paulo. In 2016, he founded the Prevent Senior Institute for the eponymous institution, with a focus on research in the field of cell therapy, and created the medical residency of that institute, an important training program for specialist doctors. In 2018 Joined the doctoral program at Nove de Julho University, with a focus on biophotonics applied to health sciences. His main interests are photobiomodulation and stem cells.

Abstract:

Timing:15:05:00 to 15:20:00 

Asthma is characterized by chronic inflammation of the airways, reversible airflow obstruction, and airway hyperresponsiveness. A common treatment is performed with the use of a corticosteroid such as Fluticasone (FT). Photobiomodulation (PBM) is relatively new, inexpensive, with no side effects, and demonstrates effectiveness in reducing inflammatory parameters. We aimed to evaluate the effects of PBM and its combination with FT in asthma. The Balb/C mice were divided into groups: controls, FT, PBM, OVA, OVA+PBM, OVA+FT, and OVA+PBM+FT. We induced inflammation by sensitization with ovalbumin - OVA and orotracheal challenge from day 21. We treated with FT (100 μg/kg - intranasal) one hour before challenge with OVA, and one hour after the challenge, we applied PBM - laser diode (660nm, 30mW, and 3J/cm²) at three distinct points. Twenty-four hours after the last treatment, the animals were anesthetized for the collection of bronchoalveolar lavage (BAL) and lungs. The data were submitted to the One-way ANOVA test, followed by the Newman-Keuls test. Significance levels adjusted to 5% (p<0.05). We observed a reduction in the total number of cells in BAL, in macrophages, with the best result in OVA+LLL, lymphocytes, with a greater reduction in the groups associated with the laser, neutrophils, and eosinophils. Reduction of production of cytokines IL-4, IL-5, IL-1β, TNF-α, and IL13, where the laser-treated groups showed better results, increased the level of the IL-10, presenting better results in the groups treated with FT. There was a reduction in collagen fiber deposition and mucus production in the airways.

Poster:

Biography:

Rita de Cássia Ferreira is a graduate nurse from UFRJ (BRAZIL) and has a Master's degree in Biophotonics Applied to Health Sciences from the University Nove de Julho (Brazil). She is currently a PhD student at the UNINOVE graduate program (Brazil). Coordinator of the Dressing Group, member of the central wound prevention and treatment committee of the Government of the State of São Paulo (Brazil) and researcher at the Biophotonics Center of the Mandaqui Hospital Complex (São Paulo, Brazil). She works in research with an emphasis on photobiomodulation in chronic and acute wounds, pressure injuries and complex wounds.

Abstract:

Timing: 15:20:00 to 15:35:00 EDT 

Fournier's gangrene (FG) is necrotizing fasciitis of the perineum, abdominal wall, and genital regions of men and women. It is characterized by obliterating endarteritis with ischemia and thrombosis of the subcutaneous vessels with necrosis and bacterial infection. 1,2 Surgical interventions accompanied by long periods of hospitalization along with coverings and dressings are the Treatment of choice for FG, making Treatment long and costly for the public health system. This study aims to present a case report on a patient with FG who underwent photobiomodulation (PBM) associated with secondary covering. Description: WOM, male, 49 years old, admitted to a public hospital in the state of São Paulo on 15 Oct. 2019 with a diagnosis of FG; emergency surgical debridement was performed accompanied by antibiotic therapy. The PBM was applied to wound with a cluster: 3 LEDs 460nm, P= 400Mw and 2 lasers 600nm, P= 100mW and 2 red LED emitters, 660nm P = 200mW; 60 seconds per points

Secondary dressing with FITOSCAR® (extract of Stryphnodendron adstringens) was changed every 48 hours for the ten days of Treatment. The lesions were evaluated using the PUSH scale with the improvement of the border, secretion, and wound center. It was concluded that PBM associated with FITOSCAR® was satisfactory in the Treatment of FG, reducing hospitalization time as well as hospital costs.

Poster: